Process for purifying pravastatin sodium from a fermentation broth
A process for purifying pravastatin sodium comprising the steps of: a. clarifying a fermentation broth containing pravastatin sodium to obtain a clear solution; and adjusting the clear solution to be basic having pH value ranging from pH 10.about.13; b. adsorbing the pravastatin sodium with non-ionic resin; eluting the pravastatin sodium by water-soluble organic solvent; and forming a concentrate of pravastatin sodium; c. treating the concentrate with water-soluble anti-solvent or inorganic salt to form a precipitate of pravastatin sodium; and d. recrystallizing the precipitate for making pravastatin sodium crystal with high purity and high yield.
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We claim:
1. A process for stably purifying pravastatin sodium comprising the steps of: a. clarifying a fermentation broth containing pravastatin sodium by a step selected from centrifugation andfiltration to obtain a clear solution containing the pravastatin sodium; and adjusting the clear solution to be basic having pH value ranging from pH 10.about.13; b. adsorbing the pravastatin sodium with non-ionic resin which is then washed with water; eluting the pravastatin sodium as adsorbed on the resin by water-soluble organic solvent, which is selected from: methanol, acetone and a mixture of methanol and acetone, to obtain pravastatin sodium fraction; and concentrating the fraction to bepravastatin sodium concentrate; c. treating the concentrate to form a precipitate of pravastatin sodium; and d. recrystallizing the precipitate for making pravastatin sodium crystal with high purity and high yield.
2. A process according to claim 1, wherein the treating of said concentrate for forming a precipitate of pravastatin sodium in step (c) comprising the addition of water-soluble anti-solvent into said concentrate.
3. A process according to claim 2, wherein said water-soluble anti-solvent selected from: ethanol, n-propanol, isopropanol, acetone and the mixture thereof.
4. A process according to claim 1, wherein the treating of said concentrate for forming a precipitate of pravastatin sodium in step (c) comprising the addition of inorganic salt for salting out the pravastatin sodium precipitate.
5. A process according to claim 4, wherein said inorganic salt is selected form: ammonium sulfate, ammonium chloride, sodium sulfate, sodium chloride and potassium chloride.
Patent number:
6790984
View patent at USPTO
Filing date:
May 14, 2003
Issue date:
September 14, 2004
Inventors:
Yu-Chin Chiang (Taichung Hsien, TW)
Chiou-Jour Laih (Taipei Hsien, TW)
Jing-Chen Mai (Taoyuan Hsien, TW)
Assignee:
Chunghwa Chemical Synthesis & Biotech Co., Ltd. (Taipei Hsien, TW)
Primary Examiner:
Paul J. Killos
Attorney, Agent or Firm:
Current U.S. Classification: 560/256
